Project

Part:BBa_I3422

Designed by: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)   Group: Antiquity   (2004-01-28)


Test: Switch response to aspartate input (I3410.E0422.I3411.E0432.I3100.I3400.I3301.I3400.I3102.I340

Tests ability of switch to respond to aspartate input by connecting the Tar-EnvZ receptor (I3301 truncated ompC upstream version) to one of the switch inputs. The remaining testbed is analogous to the switch functionality testbed (I3420).


Usage and Biology

Hk022cI switch output device, CFP reporter device, lambda cI switch output device, YFP reporter device, Lac trigger device, hk022cI switch input device, Tar-EnvZ receptor device (I3301 truncated ompC upstream version), hk022cI switch input device, araC trigger device, lambda cI switch input device


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 551
    Illegal BglII site found at 4788
    Illegal BglII site found at 5641
    Illegal BglII site found at 8395
    Illegal BamHI site found at 10096
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 715
    Illegal SapI.rc site found at 5805
    Illegal SapI.rc site found at 6265
    Illegal SapI.rc site found at 8559


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