Part:BBa_I3001

E. colibrator design 2, Polkadorks (I3410.I3200.E0422.I3510.I3401.I3411.B0015.I3100.I3701.I3300.I370

E. colibrator design 2 has an explicit OR gate before the switch via an araC OR gate device (I3701, I3700). Design 2 has no explicit activity during the switch OFF state, as illustrated by the terminator immediately following I3411 Lambda cI switch output device. This causes the OFF state to rely completely on cellular diffusion to reset the system.

Usage and Biology

The E. colibrator system is designed to create dynamic polkadots in E. coli. Cells are in one of two states stored in a bistable switch. One state, triggered by a stochastic Lac trigger or by the chemotaxis aspartate molecule, turns on a CFP reporter gene and causes synthesis and excretion of aspartate. The other state, triggered by quorum sensing under dense conditions, turns off the reporter gene and aspartate secretion. Components: Hk022cI switch output device, AspA attractant sender device, CFP reporter device, Quorum receiver device, Lambda cI switch input device, Lambda cI switch output device, double terminator, Lac stochastic trigger device, AraC input device for OR gate, Tar-EnvZ attractant receiver device, AraC buffer device for OR gate, Hk022cI switch input device, Quorum sender device

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 551
  Illegal BglII site found at 942
  Illegal BglII site found at 2407
  Illegal BglII site found at 7673
  Illegal BglII site found at 12741
  Illegal BglII site found at 13897
  Illegal BamHI site found at 12193
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 1834
  Illegal AgeI site found at 1363
  Illegal AgeI site found at 2215
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 3344
  Illegal BsaI.rc site found at 4447
  Illegal BsaI.rc site found at 13177
  Illegal SapI site found at 1874
  Illegal SapI.rc site found at 715
  Illegal SapI.rc site found at 9321
  Illegal SapI.rc site found at 12905