Measurement

Part:BBa_I23000

Designed by: Heather Keller   Group: T7.2   (2007-03-05)


RBS Scaffold with mCherry reporter

This construct is a scaffold device for measuring RBS strength via mCherry fluorescence. The device contains a ccdB expression cassette (BBa_P1010) in place of an RBS. Digestion with AvrII and SapI allows for the insertion of any RBS sequence via Heather Keller's alternative RBS assembly method, which allows for blunt cloning of an RBS upstream of a coding sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 167
    Illegal BamHI site found at 848
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 298
    Illegal SapI site found at 868


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Categories
Parameters
None