Composite

Part:BBa_I20532

Designed by: Jerome Bonnet   Group: Endy Lab   (2012-04-03)


Bxb1 BP Data Register

The DNA data register in BP and LR states consist of a constitutive promoter (BBa_J23119) flanked by BP or LR recombination sites positioned in opposite orientation, resulting in DNA inversion when recombined. A Rrnp T1 terminator (BBa_J61048) was added in reverse orientation upstream of the promoter to prevent transcriptional read-through in the opposite orientation, so that in each state, only one fluorescent protein is visibly expressed. On each side of the recombination target, we cloned superfolder GFP and mKate2 under translational control of measured strong RBSs (BIOFAB pilot C-dog project http://biofab.org/data).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 722
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1041
    Illegal NheI site found at 1064
    Illegal SpeI site found at 722
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1275
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 722
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 722
    Illegal NgoMIV site found at 730
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 750
    Illegal BsaI.rc site found at 1232
    Illegal SapI.rc site found at 1313


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