Composite
Part:BBa_I20532
Designed by: Jerome Bonnet Group: Endy Lab (2012-04-03)
Bxb1 BP Data Register
The DNA data register in BP and LR states consist of a constitutive promoter (BBa_J23119) flanked by BP or LR recombination sites positioned in opposite orientation, resulting in DNA inversion when recombined. A Rrnp T1 terminator (BBa_J61048) was added in reverse orientation upstream of the promoter to prevent transcriptional read-through in the opposite orientation, so that in each state, only one fluorescent protein is visibly expressed. On each side of the recombination target, we cloned superfolder GFP and mKate2 under translational control of measured strong RBSs (BIOFAB pilot C-dog project http://biofab.org/data).
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 722
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1041
Illegal NheI site found at 1064
Illegal SpeI site found at 722 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1275
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 722
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 722
Illegal NgoMIV site found at 730 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 750
Illegal BsaI.rc site found at 1232
Illegal SapI.rc site found at 1313
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Categories
Parameters
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