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Sample Discontinued
Experience: None
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Part:BBa_I2052

Designed by: Bartholomew Canton   Group: iGEM2006_MIT   (2007-06-15)


mCherry and GFP expression system

This system includes two fluorescent protein generators. An mCherry generating device is controlled by a LacI-repressible promoter and a GFP generating device is controlled by a T7 promoter and an orthogonal ribosome binding site.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1925
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1841


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