Measurement

Part:BBa_I2047

Designed by: Bartholomew Canton   Group: iGEM2006_MIT   (2007-06-08)


mCherry & GFP expression device for testing a biological virtual machine

mCherry expression is controlled by an e. coli promoter and ribosome binding site. The ribosome binding site is weaker than that used in either I2046 or I2045. GFP expression is controlled by an orthogonal promoter and ribosome binding site.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1895
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 167
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1811


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Parameters
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