Composite

Part:BBa_I2044

Designed by: Bartholomew Canton   Group: iGEM06_MIT   (2007-06-04)


Double protein generator (T7 RNAP and LacI)

LacI and T7 RNAP are transcribed polycistronically and translation of both is regulated by B0032. A T7 terminator is downstream. This part is being used in the construction of VM2.0

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3820
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None