Composite

Part:BBa_I2040

Designed by: Bartholomew Canton   Group: iGEM2006_MIT   (2007-05-23)


lacI repressor expression device

lacI expression is controlled by the engineered, tet repressible promoter, R0040 and a medium strength RBS. A T7 transcription terminator is used downstream which is not effective for E. coli RNAP. This terminator is being used simply because the B0032.C0013.B0016 protein generator will be used in several other constructs relating to VM2.0

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1200
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None