Reporter

Part:BBa_I2035

Designed by: Bartholomew Canton   Group: iGEM2006_MIT   (2007-05-18)


T7 RNAP dependent, lacI regulated GFP expression system

A lacI repressible T7 promoter taken from pET-11d composed with a GFP generator. The intention is to use this system to crudely characterize the behavior of the promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 799
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 715


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Categories
Parameters
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