Part:BBa_I2031:Experience
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Applications of BBa_I2031
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UNIQ10e2ecffb2849279-partinfo-00000000-QINU UNIQ10e2ecffb2849279-partinfo-00000001-QINU
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[http://2015.igem.org/Team: HKUST-Rice 2015] |
[http://2015.igem.org/Team:HKUST-Rice HKUST-Rice Team] characterized the construct BBa_I2031 on low copy plasmid pSB3K3 and high copy plasmid pSB1K3. Our results demonstrated that this promoter has a graded induction response and different sensing ranges on plasmids with different copy numbers (Figure 2). In concordance with literature, it displays an all-or-none behavior on a single cell level when expressed from a high copy plasmid pSB1K3, but not when expressed from a low copy plasmid pSB3K3 (Figure 3). Moreover, we would like to warn potential users of this part not to drive expression of riboregulators ([http://www.ncbi.nlm.nih.gov/pubmed/15208640 Isaacs et al., 2004]) using BBa_I0500. This PBAD promoter contains a 19bp sequence after the transcription start site, which would produce a taRNA with additional nucleotides at its 5’ end, and rendering it incapable to activate crRNA. To avoid misuse of this part due to incognizance of its sequence information, we recommend users to look up BBa_K1067007 for annotations. For more information and methods of our characterization, please refer to our detailed characterization report available through our [http://2015.igem.org/Team:HKUST-Rice/Expression/ParaBAD wiki page]. |