Reporter

Part:BBa_I2031

Designed by: Bartholomew Canton   Group: iGEM2006_MIT   (2007-03-28)


GFP expression device controlled by an arabinose inducible pBAD promoter

This part synthesizes GFP using a common RBS and terminator. The promoter is inducible by arabinose. This small molecule binds to the ara repressor (contained within I0500), drepressing the reporter.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1881


[edit]
Categories
//classic/reporter/pret
Parameters
None