Composite

Part:BBa_I20297

Designed by: Francois St-Pierre   Group: Endy Lab   (2010-06-21)


RBS from T7 gene 4 + Z0301 (venus)

Composite part of: (1) A relatively strong, 20 nucleotide-long RBS from phage T7 gene 4 (2) BBa_Z0301, an E.coli-optimized version of venus YFP

Note that there is no Biobrick "scar" between the RBS and Z0301

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 177
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 228


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