Coding

Part:BBa_I20285

Designed by: Jerome Bonnet   Group: Endy Lab   (2009-07-11)


Bxb1 Excisionase-Freiburg standard

This protein is a recombination directionality factor (RDF). Association with Bxb1 integrase, and triggers recombination between AttL and attR and excision of the phage genome. Bxb1 Excisionase is relatively big (28kDa) for a RDF, and is efficient both in vivo and in vitro [4]. In vitro recombination is very efficient ranging a 100% efficiency in 1hour for integration [2] and for excision [4]. Interestingly the excisionase subunit does not bind DNA and acts stoechiometrically on Bxb1 integrase [4]. Reverse inversion reaction when attL and attR in same DNA molecule. No known catalytic activity per se.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4
    Illegal AgeI site found at 772
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None