Composite

Part:BBa_I20277

Designed by: Bartholomew Canton   Group: Endy Lab   (2008-04-08)


T7 autogene

T7 RNAP transcribes itself and a LacI gene represses the same promoter. Orthogonal RBS control the expression of both genes.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2730
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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