Composite

Part:BBa_I20257

Designed by: Bartholomew Canton   Group: Endy Lab   (2007-12-07)


Autoregulated synthesis of T7 RNAP with lacI negative feedback (orthogonal translation)

An early version of a virtual machine. T7 RNAP synthesis is autoregulated. Basal amount of E. coli RNAP are needed to "boot up" the system. A lacI negative feedback loop is included to maintain a stable level of T7 RNAP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3864
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None