Generator

Part:BBa_I20253

Designed by: Bartholomew Canton   Group: Endy Lab   (2007-12-03)


Protein Generator for T7 RNAP

This protein generator uses an orthogonal RBS that is not recognized by E. coli. Use I2041 for expression of T7 RNAP in E. coli. The downstream terminator is a T7 terminator. This protein generator is designed for use in a virtual machine encoding orthogonal ribosomes and RNAP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2678
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None