Measurement

Part:BBa_I20248

Designed by: Jason Kelly, Karen Wong, Nishant Bhat   Group: Endy Lab   (2007-08-15)

Device used to characterize Ribosome Binding Sites through measurement of GFP expression

Device is based on I2055 and contains a partial biobrick site (EcoR1, Not1, Xba1) between R0040 and GFP. To insert a BB promoter for testing, digest the promoter EcoR1 and Spe1 and the kit EcoR1 and Xba1. The result will form a full BB prefix upstream of the RBS and a mixed site downstream. The full BB prefix has a larger spacing than would occur in actual construction.

Note: sequence features are wrong

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 61
    Illegal XbaI site found at 75
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 61
    Illegal NotI site found at 67
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 61
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 61
    Illegal XbaI site found at 75
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 61
    Illegal XbaI site found at 75
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 723


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