Composite

Part:BBa_I20241

Designed by: Bartholomew Canton   Group: Endy Lab   (2007-07-20)


Double protein generator of lacI and T7 RNAP

Requires a PoPS input to produce lacI and T7 RNAP on a polycistronic transcript. The same strong RBS regulates synthesis of both proteins. The downstream terminator is specific to the T7 RNAP

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3820
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None