Deleted
Sample Discontinued
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Measurement

Part:BBa_I20100

Designed by: Heather Keller   Group: Endy Lab   (2007-03-06)

RBS Scaffold with GFP reporter

All ccdb parts have been withdrawn from the Registry. Samples of parts containing the ccdB gene cannot be requested. - iGEM HQ

This construct is a scaffold device for measuring RBS strength via GFP fluorescence. The device contains a ccdB expression cassette (BBa_P1010) in place of an RBS. Digestion with AvrII and SapI allows for the insertion of any RBS sequence via Heather Keller's alternative RBS assembly method, which allows for blunt cloning of an RBS upstream of a coding sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 167
    Illegal BamHI site found at 848
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 298
    Illegal BsaI.rc site found at 1519
    Illegal SapI site found at 868


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