Project

Part:BBa_I15005

Designed by: Jeff Tabor   Group: iGEM04_UTAustin   (2004-05-26)


LacI repressed lambda cI(LVA) + LuxI(LVA) + LacZalpha

LacI repressed polycistronic cI/LuxI/LacZalpha. Requires LacZomega complementation. See notes on E0033.


Usage and Biology

We're pretty sure that E0033 is non functional. It carries a frameshifting mutation (JT 07.17.05).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1656
    Illegal BamHI site found at 1781
    Illegal XhoI site found at 1832
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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