Temporary

Part:BBa_I13771

Designed by: Jason Kelly   Group: iGEM2006_MIT   (2006-10-27)


F2621 + Codon-CBA

Construction intermediate for SP2.0, should not be used. NON-STANDARD BIOBRICK PART.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 2686
    Illegal suffix found in sequence at 2708
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2686
    Illegal NheI site found at 1167
    Illegal SpeI site found at 2709
    Illegal PstI site found at 2723
    Illegal NotI site found at 2692
    Illegal NotI site found at 2716
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2686
    Illegal BglII site found at 1203
    Illegal BamHI site found at 1924
    Illegal XhoI site found at 1181
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 2686
    Illegal suffix found in sequence at 2709
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 2686
    Illegal XbaI site found at 2701
    Illegal SpeI site found at 2709
    Illegal PstI site found at 2723
    Illegal AgeI site found at 2800
    Illegal AgeI site found at 2923
    Illegal AgeI site found at 3348
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1101
    Illegal SapI.rc site found at 2788


[edit]
Categories
Parameters
None