Temporary

Part:BBa_I13770

Designed by: Jason Kelly   Group: iGEM06_MIT   (2006-10-27)


Codon CBA

This is the sequence of the synthesized 'CBA' version of the SP2.0 synthesized by Codon Devices. It is not a standard biobrick part and should not be used. It is essentially a construction intermediate.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1520
    Illegal suffix found in sequence at 1542
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1520
    Illegal NheI site found at 1
    Illegal SpeI site found at 1543
    Illegal PstI site found at 1557
    Illegal NotI site found at 1526
    Illegal NotI site found at 1550
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1520
    Illegal BglII site found at 37
    Illegal BamHI site found at 758
    Illegal XhoI site found at 15
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1520
    Illegal suffix found in sequence at 1543
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1520
    Illegal XbaI site found at 1535
    Illegal SpeI site found at 1543
    Illegal PstI site found at 1557
    Illegal AgeI site found at 1634
    Illegal AgeI site found at 1757
    Illegal AgeI site found at 2182
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1622


[edit]
Categories
Parameters
None