Reporter

Part:BBa_I13604

Designed by: Chris Batton, Victoria Chou, Kenneth Nesmith   Group: Antiquity   (2004-07-16)

Reporter construct for constitutive YFP and inducible CFP

Reporter Construct with Tet operator+YFP(LVA-) reporter followed by a Lac operator +CFP(LVA-) reporter

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Pictures

[http://openwetware.org/wiki/IPTG IPTG] negative conditions [http://openwetware.org/wiki/IPTG IPTG] positive condition [http://openwetware.org/wiki/IPTG IPTG] positive (left) and negative (right)
BBa_I13604 visualized under non-UV light
BBa_I13604 visualized under 254nm UV lightbox
BBa_I13604 grown in IPTG visualized under 254nm UV lightbox
+(left) and -(right) [http://openwetware.org/wiki/IPTG IPTG]) visualized under 254nm wavelength UV lightbox
[edit]
Categories
//classic/reporter/multiple
Parameters
emissionYFP, CFP
excitation
tagNone