Deleted
Sample Discontinued
Experience: None
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Measurement

Part:BBa_I13581

Designed by: Reshma Shetty   Group: iGEM07_Example   (2007-07-23)

AHL-inducible promoter.S.ccdB.X.GFP

All ccdb parts have been withdrawn from the Registry. Samples of parts containing the ccdB gene cannot be requested. - iGEM HQ

This is a measurement plasmid consisting of an AHL-inducible promoter (BBa_F2620), a SpeI site, ccdB, an XbaI site, and a GFP reporter (BBa_E0840). It allows cloning of any device whose input and output are PoPS. The assembled constructs allows tunable control of the input PoPS signal (via AHL) and measurement of the output PoPS signal (via fluorescence).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1502
    Illegal SpeI site found at 1078
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 1078
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1502
    Illegal SpeI site found at 1078
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1502
    Illegal SpeI site found at 1078
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1407
    Illegal BsaI.rc site found at 1019
    Illegal BsaI.rc site found at 2173


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