Measurement

Part:BBa_I13564

Designed by: Jason Kelly   Group: iGEM2006_MIT   (2006-11-19)


Screening Plasmid 1.1

This design is identical to Screening plasmid 1.0, except the induction system (previously pBAD) has been replaced with one induced by AHL. The advantage is that this has less stringent strain requirements (no knockouts necessary). Additionally, it is available on a lower copy plasmid, psb3k3.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1855
    Illegal suffix found in sequence at 1877
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1855
    Illegal SpeI site found at 1878
    Illegal PstI site found at 1892
    Illegal NotI site found at 1861
    Illegal NotI site found at 1885
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1855
    Illegal BglII site found at 1965
    Illegal XhoI site found at 1943
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1855
    Illegal suffix found in sequence at 1878
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1855
    Illegal XbaI site found at 1870
    Illegal SpeI site found at 1878
    Illegal PstI site found at 1892
    Illegal AgeI site found at 2550
    Illegal AgeI site found at 2662
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1004
    Illegal BsaI.rc site found at 1731


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