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Part:BBa_I13563

Designed by: Jason Kelly   Group: iGEM06_MIT   (2006-11-19)


BioBrick site flanked by RNase sites and hairpins for use in Screening Plasmids

BioBrick site flanked by RNase sites and hairpins for use in Screening Plasmids

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 40
    Illegal suffix found in sequence at 62
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 40
    Illegal SpeI site found at 63
    Illegal PstI site found at 77
    Illegal NotI site found at 46
    Illegal NotI site found at 70
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 40
    Illegal XhoI site found at 128
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 40
    Illegal suffix found in sequence at 63
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 40
    Illegal XbaI site found at 55
    Illegal SpeI site found at 63
    Illegal PstI site found at 77
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None