Measurement

Part:BBa_I13546

Designed by: Jason Kelly   Group: iGEM06_MIT   (2006-09-06)

AHL-induced GFP and RFP polycistronic reporter

Note sequencing incomplete: Between 1350 and 1450 there are 11 N’s. Looking at the chromatogram by eye each one is most likely correct.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1922
    Illegal XhoI site found at 1900
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2507
    Illegal AgeI site found at 2619
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1004
    Illegal BsaI.rc site found at 1731


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