Composite

Part:BBa_I13540

Designed by: Josh Michener   Group: MIT   (2005-08-22)

Intermediate in two promoter screening plasmid

Usage and Biology

Can also be used as a GFP only control, to compare to measurements such as in pSB1A10. Transcription must be induced off of Pbad, so this part should be used in strain CW2553/pJat8 and induced at a high arabinose concentration such as 3e-4%.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1880


[edit]
Categories
//classic/composite/uncategorized
Parameters
None