Measurement

Part:BBa_I13512

Designed by: jkm   Group: iGEM04_MIT   (2005-05-31)

Screening plasmid 1

The first screening plasmid. Ends will be capped with I13450. Cutting I13452 generates an internal BioBrick site.
Background information on this screening plasmid can be found [http://openwetware.org/wiki/Endy:Screening_plasmid_1.0/Background here]
Current status on the screening plasmid project can be found [http://openwetware.org/wiki/Endy:Screening_plasmid_status here]

Usage and Biology

Note: This is NOT the most current version of the measurement device of the screening plasmid. The updated version (2.6) is available in the Registry

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1792
    Illegal AgeI site found at 1904
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2674
    Illegal SapI site found at 961


[edit]
Categories
//classic/measurement/uncategorized
Parameters
None