DNA

Part:BBa_I13452

Designed by: jkm   Group: iGEM04_MIT   (2005-05-25)


Adds another BioBrick site

Contains both a BbsI and a BstXI restriction site. When cut with both enzymes, it leaves an EcoRI and a PstI site.


Usage and Biology

Incubating BstXI at 37 C produces 50% activity (NEB). Either do a long incubation at 37 C or do a two-step incubation at 37 C, then 55 C.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//dna/cloningsite
Parameters
None