Part:BBa_I13451

Removes left Biobrick end

Contains an MfeI restriction site. Cut this part with MfeI+Spe and the other part with Eco+Xba. Ligation will form two mixed sites, neither of which can be recut. This effectively fixes the left side of the Biobrick part

Usage and Biology

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Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]