Composite

Part:BBa_I13407

Designed by: jasonk   Group: iGEM04_MIT   (2005-05-02)


YFP/CFP screening plasmid

A screening plasmid using YFP and CFP. The initial Pbad promoter has two extra restriction sites, HindIII and NcoI.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
    Illegal NheI site found at 3325
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 3264
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 3099
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961
    Illegal SapI site found at 3081


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Categories
//classic/composite/uncategorized
Parameters
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