Regulatory

Part:BBa_I13406

Designed by: jkm   Group: iGEM04_MIT   (2005-05-04)


Pbad/AraC with extra REN sites

I0500 with extra trailing restriction sites:

Eco-Xba-I0500-HindIII-NcoI-Spe-Pst

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


[edit]
Categories
//rnap/prokaryote/ecoli/sigma70
//direction/forward
//chassis/prokaryote/ecoli
//promoter
//regulation/positive
//classic/regulatory/uncategorized
Parameters
negative_regulators
positive_regulators1