Project

Part:BBa_I13404

Designed by: jkm   Group: iGEM04_MIT   (2005-04-27)


RBS.GFP + PBad CFP

Part for screening plasmid test (to be combined with an upstream inverter library).


Usage and Biology

Has a deletion at the end of the pBAD promoter. Between transcription and translation starts, so should not be significant.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2088
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2027
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1862
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 662
    Illegal SapI site found at 1844


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