Composite

Part:BBa_I13402

Designed by: jasonk   Group: iGEM04_MIT   (2005-04-26)


GFP+Pbad CFP

GFP inserted in front of Pbad CFP. Intermediate in screening plasmid construction.


Usage and Biology

Has a deletion at the end of the pBAD promoter. Between transcription and translation starts, so should not be significant.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2070
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2009
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1844
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 644
    Illegal SapI site found at 1826


[edit]
Categories
//classic/composite/uncategorized
Parameters
None