Deleted
Experience: None
Not Used
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Regulatory
Lux pL/R

Part:BBa_I13200

Designed by: SMUG Cell Signalling (Fred, Debra, Jay, Madeleine)   Group: Antiquity   (2004-07-29)


Natural LuxR/HSL Reg Promoter (Lux pL+Lux pR)

Taken from the natural system, it contains both Lux pL and Lux pR. Lux pL promotes transcription in the reverse direction and Lux pR promotes forward transcription. Contains a single base alteration at -17, what should be a T was changed to an A. It is 2 nucleotides after the "-10" region, and therefore should have a minimal overall effect on functionality.

Usage and Biology

From V. Fisheri. Promotes forward and reverse transcription under the regulation of HSL-bound LuxR. Forward trancription is activated whereas reverse transcription is repressed.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Functional Parameters

[edit]
Categories
//classic/regulatory/uncategorized
Parameters
None