Project

Part:BBa_I12028

Designed by: endelman   Group: Antiquity   (2004-07-28)


Test construct for I12006

Expression of lambda cI controlled by IPTG (requires LacIq strain). Expression of 434 cI controlled by arabinose. If I12006 promoter works, YFP is activated by lambda cI and repressed by 434 cI.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2206
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2925
    Illegal BamHI site found at 2145
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1980
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1962


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