Composite
Part:BBa_I11063
Designed by: bu Group: Antiquity (2004-09-08)
P21 half bit
The P21 half bit uses integrase/excisionase from P21 and attP/attB (rev comp) from HK022. The attachment sites are flanking a unidirectional terminator. This half bit is designed to be used with the HK022 half bit (BBa_I11062)
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 1944
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1651
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 1944
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 1944
Illegal AgeI site found at 777 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 922
[edit]
Categories
Parameters
//classic/composite/uncategorized
None |