Plasmid

Part:BBa_I11007

Designed by: Lutz and Bujard   Group: Antiquity   (2004-08-18)


pZE2 PLtetO-1 plasmid--MCS2

This plasmid contains the ColE1 origin of replication, kanamycin resistance marker, and tet0-1 promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 81
    Illegal XbaI site found at 150
    Illegal SpeI site found at 1075
    Illegal PstI site found at 126
    Illegal PstI site found at 1824
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 81
    Illegal SpeI site found at 1075
    Illegal PstI site found at 126
    Illegal PstI site found at 1824
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 81
    Illegal BglII site found at 2037
    Illegal BamHI site found at 114
    Illegal XhoI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 81
    Illegal XbaI site found at 150
    Illegal SpeI site found at 1075
    Illegal PstI site found at 126
    Illegal PstI site found at 1824
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 81
    Illegal XbaI site found at 150
    Illegal SpeI site found at 1075
    Illegal PstI site found at 126
    Illegal PstI site found at 1824
    Illegal NgoMIV site found at 1373
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1313
    Illegal SapI site found at 1523


[edit]
Categories
//classic/plasmid/uncategorized
Parameters
None