Plasmid

Part:BBa_I11006

Designed by: Lutz and Bujard   Group: Antiquity   (2004-08-18)


pZE1PLlacO-1 plasmid

This plasmid has the ColE1 origin of replication, amp resistance marker, lac0-1 promoter, and multiple cloning sites.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 81
    Illegal XbaI site found at 1765
    Illegal SpeI site found at 2690
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 81
    Illegal SpeI site found at 2690
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 81
    Illegal XhoI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 81
    Illegal XbaI site found at 1765
    Illegal SpeI site found at 2690
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 81
    Illegal XbaI site found at 1765
    Illegal SpeI site found at 2690
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2966
    Illegal SapI.rc site found at 915


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Categories
//classic/plasmid/uncategorized
Parameters
None