Plasmid

Part:BBa_I11005

Designed by: Lutz and Bujard   Group: Antiquity   (2004-08-18)


pZE1PA1lacO plasmid

Plasmid with ColE1 origin of replication, amp resistance marker, and A1lac0 promoter with multiple cloning sites

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 91
    Illegal XbaI site found at 1775
    Illegal SpeI site found at 2700
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 91
    Illegal SpeI site found at 2700
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 91
    Illegal XhoI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 91
    Illegal XbaI site found at 1775
    Illegal SpeI site found at 2700
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 91
    Illegal XbaI site found at 1775
    Illegal SpeI site found at 2700
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2976
    Illegal SapI.rc site found at 925


[edit]
Categories
//classic/plasmid/uncategorized
Parameters
None