RNA

Part:BBa_I1030

Designed by: Grace Kenney, Daniel Shen, Neelaksh Varshney, Samantha Sutton   Group: Antiquity   (2003-01-31)


RNA Regulation Target ( Promoter driven by C0040 and cI mod #1 CDS)

Coding region for the tetR promoter (BBa_C0040) with antisense binding region for BBa_I1031 and BBa_I1032, and a codon modified cI(1) protein with an LVA degradation tail.


Usage and Biology

The tetR promoter is repressed by TetR, unless TetR is bound by ATC. The resulting transcript contains a 5' "junk" region, standard RBS, and cI(1) protein with modified codon space.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 82
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//classic/rna/uncategorized
Parameters
None