Coding

Part:BBa_I10117

Designed by: Lissa Riley   Group: BE.109 2006 BE.109 Instructors   (2006-04-12)


Perforin

This is the gene for perforin. It inserts itself in to a cell's plasma membrane and causes lysis for a wide variety of cell types. It might only work if granzyme is also present.

The protein is 555 amino acids long.

The gene contains 3 exons.

After *extensive* searching, there appears to be disagreement on the ORF length. Several sources cite a transcript of length ~4 kbp, but ORF Finder gives nothing longer than ~1.2 kbp.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 408
    Illegal NgoMIV site found at 710
    Illegal NgoMIV site found at 714
    Illegal NgoMIV site found at 892
    Illegal NgoMIV site found at 955
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 833
    Illegal SapI site found at 1110


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Categories
Parameters
None