Part:BBa_I10088
Cyanobacterial Nitrogenase Coding Region
This gene isolated from species of the genus Anabaena encodes the protein nitrogenase, which degrades cyanide and generates methane and ammonia. This part would theoretically be a preliminary part, which could be subjected to experiments in which it would be cut at various sites. The goal of these experiments would be to isolate the part of the gene which would halt the cyanide-> methane + ammonia reaction at the phase of the reaction's intermediates, namely imines and hydrogen-based compounds. This piece of the coding region could then be used to create a cyanide-degrading part which does not release the hazardous methane and ammonia products.
Usage and Biology
Although this part has never been used by people involved in the Registry, it has been isolated in many laboratories, and extensive studies have been done regarding the efficiency of the nitrogenase enzyme product in converting cyanide into methane and ammonia.
See:
"The heterocyst-specific fdxH gene product of the cyanobacterium Anabaena sp. PCC 7120 is important but not essential for nitrogen fixation." (1997) by Masepohl B, Scholisch K, Gorlitz K, Kutzki C, Bohme H in Mol Gen Genetics
"Biological Degradation ofCyanide by Nitrogen- Fixing Cyanobacteria." (1990) by C. Gantzer and W. Maier
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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