Coding

Part:BBa_I10060

Designed by: Sasha Brophy   Group: BE.109 2006 BE.109 Instructors   (2006-04-21)


-- Mab G10/4 --


-- This part is a monoclonal antibody G10/4 that binds to variant specific surface protein H7 in protozoan Giardia lamblia. Giardia lamblia is a pathogen found in water that infects the gastrointestinal tract of humans. The sequence was found at http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=159142. The sequence information was found by researching the antibodies that bind to the surface protein of Giardia lamblia at Pubmed.gov. The PstI site was removed from the sequence with the gene design site http://slam.bs.jhmi.edu/gd/ so it can be compatible with the BioBrick ends. --

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 742
    Illegal NheI site found at 877
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 107
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None