Coding

Part:BBa_I10001

Designed by: Chris Anderson (Voigt Lab)   Group: BE.109 2006 BE.109 Instructors   (2006-04-04)
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Safety Flag

The iGEM Safety Committee has placed a Red Flag on this part. This part presents safety risks beyond what is normal for the Registry. Researchers who plan to acquire and use this part should take special care to ensure they use it safely and responsibly. Contact safety (AT) igem (DOT) org with any questions.

Reason: Listeriolysin and Invasin parts

If you are an iGEM team, you must submit a Check-In before acquiring and using this part! See the 2017 Safety Page for more information.


Invasin

Invasin is a bacterial membrane protein (from Y.pseudotuberculosis) that binds tightly to beta-1-integrins found on the surface of many mamallian cells; interaction between invasin and beta-1-integrins induces the physical uptake of bacteria by mamallian cells via a Rac-1 mediated response (Wong & Isberg, 2005; PMID: 15694850)


Usage and Biology

Chris Anderson & colleagues have demonstrated that the display of invasin on E. coli allows invasin-mediated internalization by mammalian cells; such update does not require additional known adhesion molecules. Their work was done in E. coli MC1061, a fim+ strain, and CAMC600, a fim- strain (Andereson et al., 2005 PMID: 16330045). Inv+ E. coli can invade a broad range of tumor cells (epithelial, hepatocarcinoma, and osteosarcoma).

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 840
    Illegal AgeI site found at 349
    Illegal AgeI site found at 673
    Illegal AgeI site found at 808
    Illegal AgeI site found at 886
    Illegal AgeI site found at 2395
    Illegal AgeI site found at 2899
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None