Primer
BioBrick-r

Part:BBa_G1005

Designed by: Reshma Shetty   Group: iGEM07_Example   (2008-10-17)


Reverse BioBrick suffix primer for amplifying BioBrick parts (BioBrick-r)

Reverse primer that binds to the BioBrick suffix. It is useful for amplifying BioBrick parts.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 23
    Illegal PstI site found at 9
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 23
    Illegal PstI site found at 9
    Illegal NotI site found at 14
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 23
    Illegal PstI site found at 9
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 23
    Illegal PstI site found at 9
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//primer/part/amplification
Parameters
directionR
len_end
len_fr
len_start
pct_gc55.2
targetBioBrick plasmid backbones
t_m64.7