Primer
BioBrick-r
Part:BBa_G1005
Designed by: Reshma Shetty Group: iGEM07_Example (2008-10-17)
Reverse BioBrick suffix primer for amplifying BioBrick parts (BioBrick-r)
Reverse primer that binds to the BioBrick suffix. It is useful for amplifying BioBrick parts.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 23
Illegal PstI site found at 9 - 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 23
Illegal PstI site found at 9
Illegal NotI site found at 14 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 23
Illegal PstI site found at 9 - 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 23
Illegal PstI site found at 9 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//primer/part/amplification
direction | R |
len_end | |
len_fr | |
len_start | |
pct_gc | 55.2 |
target | BioBrick plasmid backbones |
t_m | 64.7 |