Primer
BioBrick-f

Part:BBa_G1004

Designed by: Reshma Shetty   Group: iGEM07_Example   (2008-10-17)


Forward BioBrick prefix primer for amplifying BioBrick parts (BioBrick-f)

Forward primer that binds to the BioBrick prefix. It is useful for amplifying BioBrick parts.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 9
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 9
    Illegal NotI site found at 15
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 9
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 9
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 9
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//primer/part/amplification
Parameters
directionF
len_end
len_fr
len_start
pct_gc53.6
targetBioBrick plasmid backbones
t_m63.0