Primer
Suffix-F

Part:BBa_G1000

Designed by: Jason Kelly   Group: iGEM06_MIT   (2008-04-22)


Forward primer for amplifying BioBrick plasmid backbones by PCR (Suffix-f)

This is the FWD primer used in the measurement kit to amplify pSB3K3

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//primer/plasmid/amplification
Parameters
directionF
len_end
len_fr
len_start
pct_gc61.9
targetBioBrick plasmid backbones
t_m61.3