Primer
GFP-r

Part:BBa_G00201

Designed by: Bartholomew Canton   Group: iGEM06_MIT   (2007-03-01)


Reverse primer for sequencing composite parts; binds to GFP (BBa_E0040)

This is a reverse primer that bind about midway through the GFP coding sequence and extends towards the start codon.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//primer/reporter/GFP
Parameters
directionR
len_endn/a
len_frn/a
len_start305
pct_gc50
targetBBa_E0040
t_m62